Human FGF-17 Protein, His Tag (HPLC verified)

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FG7-H5144-20ug
$145.00
FG7-H5144-50ug
$215.00
FG7-H5144-500ug
$1300.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    FGF-17, Fibroblast growth factor 17, FGF17

  • Source

    Human FGF-17 Protein, His Tag (FG7-H5144) is expressed from E. coli cells. It contains AA Thr 23 - Thr 216 (Accession # O60258-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    FGF-17 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 24.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Host Cell Protein

    <0.5 ng/µg of protein tested by ELISA.

  • Host Cell DNA

    <0.02 ng/μg of protein tested by qPCR.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    FGF-17 SDS-PAGE

    Human FGF-17 Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    FGF-17 SEC-HPLC

    The purity of Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     FGF-17 ELISA

    Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 2-40 ng/mL (QC tested).

    Protocol
  •  FGF-17 ELISA

    Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with a linear range of 0.078-0.625 μg/mL (QC tested).

    Protocol
  • Bioactivity-CELL BASE

     FGF-17 CELL

    Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) stimulates proliferation of NIH-3T3 cells. The EC50 for this effect is 1.495-1.639 µg/mL (Routinely tested).

    Protocol

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