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Human Fc Receptor Blocking Solution Protein, Tag Free

COADS MS
DS

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제품 번호/규격
가격
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FCN-H5213-50tests
$75.00
FCN-H5213-200tests
$165.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0
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제품 세부정보

  • Synonyms

    Fc Receptor Blocking Solution

  • Source

    Human Fc Receptor Blocking Solution Protein, Tag Free (FCN-H5213) is expressed from human 293 cells (HEK293).

  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 31.2 kDa.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • Bioactivity-FACS

    Fc Receptor Blocking Solution FACS

    Flow cytometric analysis was performed on THP-1 cells that were either treated with Human Fc Receptor Blocking Solution Protein, Tag Free (Cat. No. FCN-H5213) using a 1:20 dilution (5 μL of antibody stock per 1e6 cells in a final volume of 100 µL) or left untreated. All cells were stained with an irrelevant PE-conjugated mouse IgG2a antibody. PE signal was used to evaluate the blocking effect (QC tested).

    Protocol

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배경 소개

Fc receptors expressed on the surface of immune cells such as monocytes, macrophages, dendritic cells, and certain lymphocyte subsets can bind to the Fc region of antibodies in a non‑antigen‑specific manner. This Fc–Fc receptor interaction often leads to high background signals in flow cytometry, immunofluorescence, and other immunoassays, complicating the interpretation of true antigen‑specific binding. To address this, Fc receptor blocking reagents are used prior to antibody staining to occupy Fc receptors, thereby preventing non‑specific binding and improving assay specificity and signal‑to‑noise ratio. The blocking efficiency may vary depending on the antibody isotype, Fc receptor subtype, and cell type tested.

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