Mouse ENPP3 Protein, Fc Tag (active enzyme, MALS verified)

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제품 번호/규격
가격
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EN3-M5263-100ug
$360.00
EN3-M5263-1mg (500ug X 2)
$2380.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    ENPP3, CD203c, NPP3, E-NPP3, PD-Ibeta, NPPase, PDNP3

  • Source

    Mouse ENPP3 Protein, Fc Tag (EN3-M5263) is expressed from human 293 cells (HEK293). It contains AA Arg 46 - Ile 874 (Accession # Q6DYE8-1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    ENPP3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 120.3 kDa. The protein migrates as 130-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • SDS-PAGE

    ENPP3 SDS-PAGE

    Mouse ENPP3 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    ENPP3 SEC-MALS

    The purity of Mouse ENPP3 Protein, Fc Tag (Cat. No. EN3-M5263) is more than 90% and the molecular weight of this protein is around 245-285 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to hydrolyze the Pyrophosphate (PPi) from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 700 pmol/min/μg (QC tested).

    Protocol

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배경 소개

The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    4 상세

  • 최고 개발 단계:

    Phase 1 Clinical

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