Endo H (500U/μl)

Product Details

Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins.

Application

Endo H cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.

Unit Definition

One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.

Purity

>95% as determined by SDS-PAGE.

Enzyme Activity

>500 U/μL

Formulation

Supplied as 0.2 μm filtered solution in 20 mM Tris, 50 mM NaCl, pH7.5.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

This product is stable after storage at:

1. The product MUST be stored at -20°C or lower upon receipt.
2. -20°C for 12 months under sterile conditions.