Chikungunya virus (CHIKV) E2 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
E2P-V52H3-50ug
$472.00
E2P-V52H3-500ug
$2940.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    E2

  • Source

    Chikungunya virus (CHIKV) E2 Protein, His Tag (E2P-V52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 326 - Gln 666 (Accession # Q1H8W5).

    Predicted N-terminus: Ser 326

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  • Molecular Characterization

    E2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.3 kDa. The protein migrates as 48-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    E2 SDS-PAGE

    Chikungunya virus (CHIKV) E2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    E2 SEC-MALS

    The purity of Chikungunya virus (CHIKV) E2 Protein, His Tag (Cat. No. E2P-V52H3) is more than 85% and the molecular weight of this protein is around 38-58 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     E2 ELISA

    Immobilized Chikungunya virus (CHIKV) E2 Protein, His Tag (Cat. No. E2P-V52H3) at 1 μg/mL (100 μL/well) can bind CHIKV E2 Antibody (8E12) Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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Background

The envelope protein 2 (E2) of Chikungunya virus (CHIKV) is a key surface glycoprotein responsible for host cell receptor binding and viral entry. It forms heterodimers with the E1 fusion protein and assembles into trimeric spikes on the viral surface. E2 contains major neutralizing epitopes, making it a prime target for vaccine development and serological diagnostics.

Recent Advances

 
Drug Development Progress
  • English Name:

    Human papillomavirus regulatory protein E2

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Pending

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