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Synonyms
DM, DM1, DM1PK, DMK, MDPK, MT-PK
Source
Human DMPK Protein, His Tag (DMK-H5143) is expressed from E. coli cells. It contains AA Leu 10 - Pro 420 (Accession # Q09013-9).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 48.0 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Human DMPK Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DMPK Protein, His Tag (Cat. No. DMK-H5143) is more than 90% and the molecular weight of this protein is around 86-100 kDa verified by SEC-MALS.
Report
Bioactivity
The DMPK assay is performed using the ADP-GloTM Kinase Assa kit which quantifies the amount of ADP produced by the DMPK reaction. The ADP-GloTM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction. The specific activity is >2 pmol/min/ug (QC tested).
Protocol
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