제품 수집
제품 세부정보
Synonyms
Delta3, delta-like 3 (Drosophila), delta-like protein 3, DLL3, Pudgy, SCDO1, SCDO1delta3
Source
Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (DL3-HA2H3) is produced via conjugation of AF647 to Human DLL3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human DLL3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).
Predicted N-terminus: Ala 27
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
Evaluation of CAR expression
FACS Analysis of Anti-DLL3 CAR Expression
5e5 of anti-DLL3 CAR-293 cells were stained with 100 μL of 1 μg/mL of Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (Cat. No. DL3-HA2H3) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa 647 signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (Cat. No. DL3-HA2H3) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Delta-like protein 3
- 카테고리：
- 상장 의약품 수：
1 상세
- 임상 의약품 수：
28 상세
- 최고 개발 단계：
Approved
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전화
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