제품 수집
제품 세부정보
Synonyms
Complement C5, C5, CPAMD4
Source
Cynomolgus Complement C5, His Tag (CO5-C52Hx) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # XP_005580972.1).
Predicted N-terminus: Gln 19 (β chain) & Met 678 (α chain)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 65 kDa and 113 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Cynomolgus Complement C5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus Complement C5, His Tag (Cat. No. CO5-C52Hx) is more than 85% and the molecular weight of this protein is around 175-215 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus Complement C5, His Tag (Cat. No. CO5-C52Hx) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Complement C5, His Tag (Cat. No. CO5-C52Hx) at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
사용자 리뷰 댓글 남기기
1***32022-08-10
좋아요I tried to express this product myself and it didn’t work, so I purchased ACRO’s products for antibody screening. The binding activity is very good and method establishment is also going very smoothly. There is also complete technical support available to help us solve any problems that arise during our experiments.
배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Complement C5
- 카테고리：
- 상장 의약품 수：
8 상세
- 임상 의약품 수：
20 상세
- 최고 개발 단계：
Approved
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전화
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