PE-Labeled Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag (Nanodisc) (Site-specific conjugation)

Application

1. Cell based assay (FACS or functional assay)
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

Claudin-18.2, CLDN18, Claudin-18

Source

Nanodisc PE-Labeled Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag (CL2-HP2D3) is produced via site-specific conjugation of PE to Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag under optimal conditions with a proprietary technology. Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 200 (Accession # P56856-2). In the region Met 1 - Ala 200, the amino acid sequences of Human and Cynomolgus Claudin-18.2 are identical.

Predicted N-terminus: Met

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the N-terminus and a Flag tag at the C-terminus. The protein has a calculated MW of 26.8 kDa.

*The isotype control of empty/mock nanodisc (Cat. No. APO-HP1E3) is sold separately and not included in protein, you can follow the link for product information.

1. Structure

   

   Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

2. Synthesis Process

   

   Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Formulation

Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process