제품 수집
제품 세부정보
Synonyms
CD19, B4, CVID3, MGC12802
Source
PE-Labeled Human CD19 (20-291), His Tag (CD9-HP2H3) is produced via site-specific conjugation of PE to Human CD19 (20-291), His Tag under optimal conditions with a proprietary technology. Human CD19 (20-291), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
Predicted N-terminus: Pro 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.7 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Evaluation of anti-CD19 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
FACS Analysis of Blood Samples
After Fc receptor blockade, whole blood only control (Figure A) and whole blood spiked with CAR-T cells (Figure B) were first stained with PE-Labeled Human CD19 (20-291) Protein, His Tag (Cat. CD9-HP2H3), following 7-AAD staining. The data were analyzed using FCS Express 7 software (Routinely tested). CAR-T cells were identified as PE-positive events within the live CD45+CD3+ (or CD45+) lymphocyte population. The proportion of CD45+CAR+ cells is around 20%, which is largely consistent with the theoretical spike-in value.
Protocol
Bioactivity-Flow Cytometry
1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3) (QC tested).
Protocol
사용자 리뷰 댓글 남기기
P***y2023-06-20
좋아요We use this reagent to detect CD19 CAR on the cell surface by flow cytometry. We use it at the recommended dose and are very happy with the results.
- S*** 2023-02-01 좋아요I run lentiviral titers at work and desperately needed a CD19 antibody due backorder. I stumbled upon Acro Bio's PE-CD19 abs and it worked wonders! When I first used it, there was higher CD19 expression than I typically see in my vectors. I performed a comparison study testing out various CD19 (some conjugated some not conjugated) antibodies. The Acro Bio antibody had the best performance! It showed greater binding than the usual CD19 antibody I use. After I conducted this study, my company set their heart's desire to Acro's CD19 antibody. We solely use this antibody for all our CD19 projects. AND its more affordable than many other CD19 antibodies on the market! This is such a phenomenal antibody! I highly recommend it!
- R***t2023-01-04 좋아요Thank you so much ACRO for your CD19 materials. I work in a CAR focused lab and the CD19 we get from you is instrumental to our operations. I work in immunology and this antibody is used in all my downstream flow assays to check the status of our novel CAR materials. I use it at least weekly, and the staining is easy and immaculate. My peaks are clear and I couldn't be happier.
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
B-lymphocyte antigen CD19
- 카테고리：
- 상장 의약품 수：
14 상세
- 임상 의약품 수：
435 상세
- 최고 개발 단계：
Approved
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