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Product Details
Synonyms
ICE, IL1BC, P45
Source
Human Caspase-1 Protein, His Tag&Tag Free (CA1-H51W3) is expressed from E. coli cells. It contains AA Asn 120 - Asp 297 & Ala 317 - His 404 (Accession # P29466-1).
Predicted N-terminus: Met & Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.9 kDa & 10.4 kDa. The protein migrates as 9 kDa and 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.5 M Arginine, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Caspase-1 Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
Measured by its ability convert the substrate acetyl-Tyr-Val-Ala-Asp p-nitroanilide (Ac-YVAD-pNA) to p-nitroaniline (pNA). The specific activity is >50 pmol/min/µg (QC tested).
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