Human CA125 / MUC16 (13810-14451) Protein, His Tag, low endotoxin (Mass Spec & MALS verified)

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CA5-H52H6-100ug
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CA5-H52H6
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Synonyms

CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, mucin-16

Source

Human CA125 (13810-14451), His Tag (CA5-H52H6) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).

Predicted N-terminus: Pro 13810

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 73.7 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry
  • Sequence Coverage

    Human CA125 (13810-14451), His Tag(CA5-H52H6) was digested with chymotrypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

배경 소개

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

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