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Product Details
Synonyms
Activin E
Source
Human Activin E Protein, His Tag (INE-H5143) is expressed from E. coli cells. It contains AA Thr 237 - Ser 350 (Accession # P58166).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
Mass Spectrometry
Measured Molecular Mass
The molecular weight by mass spectrometry is 28951 Da (Routinely tested).
Sequence Coverage
Human Activin E Protein, His Tag(INE-H5143) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
N-terminal Sequence Analysis
Met-(Tag)-Thr-Pro-Thr-Cys-Glu-Pro-Ala-Thr-Pro-Leu-Cys-Cys-Arg-Arg-Asp
(Routinely tested)
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Activin E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Activin E Protein, His Tag (Cat. No. INE-H5143) is more than 90% and the molecular weight of this protein is around 27-35 kDa verified by SEC-MALS.
Report
Mass Spectrometry
ESI-MS analysis of Human Activin E Protein, His Tag (Cat. No. INE-H5143), showing a major peak at 28951 Da, which consistent with the native homodimer. The minor peak following the major peak is a matrix-associated artifact of the ESI-MS (Routinely tested).
Bioactivity-ELISA
Immobilized Human Activin E Protein, His Tag (Cat. No. INE-H5143) at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus Activin RIIA / ACVR2A Protein, Fc Tag (Cat. No. ACA-H5269) with a linear range of 0.004-0.06 μg/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Human Activin E Protein, His Tag (Cat. No. INE-H5143) induced ALK-7 reporter activity in HEK293 cells. The EC50 for this effect is 0.9882 ng/mL (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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