Human Activin E Protein, His Tag (MS verified)

Synonyms

Activin E

Source

Human Activin E Protein, His Tag (INE-H5143) is expressed from E. coli cells. It contains AA Thr 237 - Ser 350 (Accession # P58166).

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.5 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

The protein is designed as a dimer.

Mass Spectrometry

1. Measured Molecular Mass

   The molecular weight by mass spectrometry is 28950.2 Da.

2. Sequence Coverage

   Human Activin E Protein, His Tag (MS verified)(INE-H5143) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage.

3. N-terminal Sequence Analysis

   (Tag)-Thr-Pro-Thr-Cys-Glu-Pro-Ala-Thr-Pro-Leu-Cys-Cys-Arg

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process