resDetect™ Human Fibronectin ELISA Kit (Residue Testing)

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제품 번호/규격
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수량
RES-A108-96tests
$320.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    Fibronectin
    Format
    96T (8×12 strips)
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    <100 pg/mL
    Standard Curve Range
    100 pg/mL-3200 pg/mL
    Assay Time
    3 hr 20 min
    Suitable Sample Type
    For the detection and quantitative determination of Human Fibronectin in cell culture supernates.
    Sample volume
    100 uL

  • Product Overview

    Fibronectin, a vital extracellular matrix protein, significantly impacts cell adhesion, spreading, migration, tissue repair, cell shape maintenance, and cell signaling. It exists as a dimer with disulfide - linked polypeptide chains and multiple functional domains. Different isoforms, like soluble and cell - associated ones, play distinct roles in wound healing, blood clotting, and cell - matrix interactions.

    To support the development of cell therapy drugs, ACROBiosystems developed Human fibronectin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of fibronectin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of Recombinant Fibronectin fragment, premium grade (ACROBiosystems, cat# GMP-FINH18) concentrations.

  • Application

    The kit is developed for the detection and quantitative determination of fibronectin in cell culture supernates.

    It is for research use only.

  • Storage

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    RES108-C01
    Pre-coated Anti-Human Fibronectin Antibody Microplate
    1 plate(8×12 strips)
    RES108-C02
    Human Fibronectin Standard
    5 μg×2
    RES108-C03
    Biotin-Anti-Human Fibronectin Antibody
    20 μg
    RES108-C04
    Streptavidin-HRP
    50 μL
    RES108-C05
    10×Washing Buffer
    50 mL
    RES108-C06
    2×Dilution Buffer
    50 mL
    RES108-C07
    Substrate Solution
    12 mL
    RES108-C08
    Stop Solution
    7 mL

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Typical Data 

    Please refer to DS document for the assay protocol.

     Fibronectin TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
  • Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of human Fibronectin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

     Fibronectin DILUTION LINEARITY
  • Intra-Assay Statistics

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

     Fibronectin INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

     Fibronectin INTER-ASSAY STATISTICS
  • Recovery

    Five parts of blank T cell culture supernatant were added with different concentrations of human Fibronectin, and the T cell culture supernatant without human Fibronectin was used as background to calculate the recovery rate. The range of the recovery rate is 82.5-93.7%, and the average recovery is 89.3%.

     Fibronectin RECOVERY

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