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The 293T resDNA Quantitation Kit is designed for the quantitative detection of residual 293T host cell DNA in biomanufacturing processes that employ the 293T cell line, such as the production of viral vectors for gene therapy, CAR-T cell products, recombinant proteins, antibodies, and viral vaccines. Based on real-time quantitative PCR (qPCR) technology, this kit delivers sensitive, specific, and reproducible quantification of residual 293T DNA with a lower limit of quantitation (LLOQ) of 3 fg/µL. This kit is applied after host cell DNA isolation from test samples. For improved DNA recovery and consistent performance, it is recommended to use the kit together with the HEK293 / HEK293T resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R033).
The kit is used for quantitative detection of residual Human DNA in biopharmaceuticals.
For use in quality control/manufacturing process only.
It is for research use only.

If your experimental workflow involves host cell DNA sample preparation, please use the recommended sample preparation kits listed in the table. This ensures that the buffers used during both sample preparation and residual DNA quantitation remain consistent, supporting reliable and accurate results.

ID | Components | Size |
OPA-R032-01 | 2×qPCR Master Mix | 1.0 mL×2 |
OPA-R032-02 | HEK293T Primer & Probe Mix | 700 μL |
OPA-R032-03 | HEK293T DNA Control(3ng/μL) | 100 μL |
OPA-R032-04 | Dilution Buffer | 1.5 mL×3 |
OPA-R032-05 | DNase/RNase-Free Water | 1.0 mL |