resDetect™ HEK293T resDNA Ultra-Low Quantitation Kit (qPCR)

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OPA-R032-100tests
$1,933.00
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OPA-R032
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Product Overview

The 293T resDNA Quantitation Kit is designed for the quantitative detection of residual 293T host cell DNA in biomanufacturing processes that employ the 293T cell line, such as the production of viral vectors for gene therapy, CAR-T cell products, recombinant proteins, antibodies, and viral vaccines. Based on real-time quantitative PCR (qPCR) technology, this kit delivers sensitive, specific, and reproducible quantification of residual 293T DNA with a lower limit of quantitation (LLOQ) of 3 fg/µL. This kit is applied after host cell DNA isolation from test samples. For improved DNA recovery and consistent performance, it is recommended to use the kit together with the HEK293 / HEK293T resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R033).

Features
  • Ultra-high sensitivity for optimal product safety: Stable detection of HEK293T host cell residual DNA down to 3 fg/μL
  • High specificity: No cross-reactivity with unrelated DNA
  • Regulatory compliance: ICH Q2(R2) & FDA Bioanalytical Method Validation compliant
  • Premium quality: Manufactured in ISO 13485 certified facility
Application

The kit is used for quantitative detection of residual Human DNA in biopharmaceuticals.

For use in quality control/manufacturing process only.

It is for research use only.

Technical Specifications
DNA Technical Specifications
Attention

If your experimental workflow involves host cell DNA sample preparation, please use the recommended sample preparation kits listed in the table. This ensures that the buffers used during both sample preparation and residual DNA quantitation remain consistent, supporting reliable and accurate results.

DNA Attention
Materials Provided
ID
Components
Size
OPA-R032-01
2×qPCR Master Mix
1.0 mL×2
OPA-R032-02
HEK293T Primer & Probe Mix
700 μL
OPA-R032-03
HEK293T DNA Control(3ng/μL)
100 μL
OPA-R032-04
Dilution Buffer
1.5 mL×3
OPA-R032-05
DNase/RNase-Free Water
1.0 mL