HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line

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CHEK-ATP132-2Vials (1Vial X 2)
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Advantages
  • Genetically modified cell lines best reflect MOA (Mechanism of Action)
  • Higher activity and larger assay window for robust and reproducible cell-based bioassay
  • Comprehensive application data to support assay development and validation
  • Full tracible record, stringent quality control and validated cell passage stability
  • Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  • Global commercial license assistance whenever regulatory filing is required
Description

HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line stably expressing exogenous human FCGRT (Uniprot: P55899) & B2M (Uniprot: P61769). At the same time, GFP protein as a marker was expressed in HEK293/FcRn (FCGRT & B2M), GFP Tag Cells for screening positive cells by FACS.

Application

• Useful for cell-based FcRn (FCGRT & B2M) binding assay.

Growth Properties

Adherent

Selection Marker

Zeocin (25 μg/mL) + Puromycin (5 μg/mL)

Complete Growth Medium

DMEM + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.