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Product Details
- ProductSizeAmountCellectra™ Human TCRαβ NanoDeplet Microbeads, Research grade2 mLFor 10⁹ total cells
Product Description
Cellectra™ Human TCRαβ NanoDeplet Microbeads, Research grade are dextran-coated superparamagnetic iron oxide nanoparticles of which the surface is conjugated with recombinant monoclonal antibodies specific to human TCRαβ (Isotype: mouse IgG1). They are especially designed for the depletion of TCRαβ+ cells.
Cellectra™ Human TCRαβ NanoDeplet Microbeads, Research grade are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
Cellectra™ Human TCRαβ NanoDeplet Microbeads, Research grade are designed for the depletion of TCRαβ⁺ cells from fresh or frozen human peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cells (CBMCs) or other cell products. TCRαβ represents the hallmark receptor complex on nearly all adaptive CD4 helper and CD8 cytotoxic T cells, and functions as the primary effector marker driving graft-versus-host disease (GVHD) and allogeneic rejection. Conventional CD3 depletion reagents remove all T cell subsets indiscriminately. In contrast, this microbead product enables targeted elimination of solely TCRαβ+ adaptive T cells, completely retaining intact populations of γδ T cells, NK cells, B cells, monocytes and dendritic cells for unconfounded innate immune research. The magnetic Microbeads are conjugated with monoclonal antibodies targeting human TCRαβ protein, enabling TCRαβ+ cells to be labeled with the specific antibodies and magnetic particles, and separated using the separation columns and magnets.
Storage
For long term storage, the product should be stored in liquid state at -20°C or below Stability upon receipt: Stable for 12 months at -20 °C or below; Stable for 3 months at 2–8 °C under sterile conditions.
Please protect from light and avoid repeated freeze-thaw cycles.
Sterility
Negative
Endotoxin
Less than 2 EU per mL by the LAL method / rFC method.
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Supplied in PBS buffer containing EDTA, rHSA and Poloxamer 188.
ACRO Quality Management System
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Performance Data
Application Data
Superior the depletion Performance of TCRαβ+ Cells (Comparison with Company M)
Separation performance was evaluated using samples containing 1×10⁷ human PBMCs, processed with TCRαβ microbeads from Acro, Company M at recommended dosages. Negative fractions were collected, and 1×10⁶ cells from the flow through fraction were immunostained with TCRαβ Antibody (FITC-anti human TCRαβ Antibody and PE anti-human CD3 Antibody) and 7-AAD, followed by flow cytometric analysis. Data indicated that Acro microbeads achieved a final purity of 99.03% (baseline: 46.01%). These findings validate outstanding separation performance, confirming an optimal balance of purity and recovery relative to Company M benchmarks.
Comparison of the depletion of TCRαβ+ Cells Separation Performances
Isolation efficacy was validated using samples containing 1×10⁷ human PBMCs processed with the recommended dosage of Cellectra™ Human TCRαβ NanoDeplet Microbeads, Research grade, in parallel with Company M. Comparative analysis demonstrated that Cellectra™ Human TCRαβ NanoDeplet Microbeads achieved higher cell recovery rates than Company M, while purity and cell viability were comparable to Company M.
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