Monoclonal Anti-SN38 Antibody, Mouse IgG1

Immobilized Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) at 5 μg/mL, add Sacituzumab Govitecan in the 50% Mouse serum and then add Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

Immobilized Sacituzumab Govitecan at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with a linear range of 0.12-0.98 ng/mL (Routinely tested).

ELISA binding of Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with Trastuzumab Deruxtecan, Sacituzumab Govitecan, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively. The coating antibody was Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Trastuzumab Deruxtecan, Sacituzumab Govitecan, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) (Jackson, Cat. No. 109-035-098) used at 1:12000 concentration. Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) is specific to Sacituzumab Govitecan and has no cross-reactivity with Trastuzumab Deruxtecan, Trastuzumab MMAE and Trastuzumab-DM1 (Routinely tested).