Recombinant Monoclonal Anti-PD-L1 Antibody, Rabbit (5D3)

제품 세부정보

Background
Recombinant Monoclonal Anti-PD-L1 Antibody, Rabbit (5D3) is a PD-L1 primary antibody for IHC application. PD-L1, is a ligand that binds with the receptor PD1, commonly found on T-cells, and acts to block T-cell activation. PD1 expression has been observed in a variety of cancers including melanoma and non-small cell lung cancer. The interaction of PD1/PDL1 is hypothesized to be a possible mechanism for the tumor to escape immune response. A number of checkpoint blockade inhibitors including pembrolizumab and nivolumab have been developed that target the PD1/PDL1 interaction in order to allow T-cells to recognize tumor cells without being deactivated by the tumor.

Host Species	Rabbit
Isotype	Rabbit IgG, Rabbit Kappa
Clone	5D3
Application	IHC-P
Dilution Ratio	1: 500 - 1: 2000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	CD274, PD-L1
Concentration	0.9~1.1 mg/ml
Specificity	React with PD-L1 protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human small cell lung cancer tissue labelled with HCS-S240 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human liver cancer tissue labelled with HCS-S240 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human colorectal cancer tissue labelled with HCS-S240 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human esophageal cancer tissue labelled with HCS-S240 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human gastric cancer tissue labelled with HCS-S240 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.

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