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Application
The Monoclonal Anti-Mouse IgG Antibody Europium chelate is a universal tool for TR-FRET assays that can bind mouse IgG or mFc-tagged proteins. This product uses specific anti-mouse IgG antibody covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-Mouse IgG Antibody is a rabbit monoclonal antibody which recognizes mouse immunoglobulins with high affinity.SpecificityThis antibody recognizes mouse IgG1, IgG2a, IgG2b and shows lower reactivity toward IgG3.Cross-ReactivityCross-reactivity with rat IgG, whereas no reactivity to human IgG, goat IgG, rabbit IgG or chicken IgY.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the mouse IgG antibody or mouse Fc tagged protein as acceptor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Monoclonal Anti-Mouse IgG Antibody Europium chelate (Cat. No. FRT-TD03) is used as the Donor and Streptavidin Protein FA-Labeled (Cat. No. FRT-TA01) is used as the Acceptor. Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6), at 50 ng/well, can bind to the Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear detection range of 5-0.01 μg/mL (QC tested).
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