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Product Details
Application
The Monoclonal Anti-Flag Tag Antibody Europium chelate is a universal tool for TR-FRET assays that can bind Flag-tagged proteins. This product uses specific anti-Flag Tag antibody covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-Flag Tag Antibody, Rabbit IgG is a rabbit monoclonal antibody recombinantly expressed from HEK293.SpecificityThis antibody specifically reacts with Flag tag proteins, and can recognizes C-terminal and N-terminal Flag tagged fusion proteins.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the flag-tagged protein as Donor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Monoclonal Anti-Flag Tag Antibody Europium chelate (Cat. No. FRT-TD06) is used as the Donor and Streptavidin Protein FA-Labeled (Cat. No. FRT-TA01) is used as the Acceptor. Biotinylated Human CD40 Protein, Fc,Avitag (Cat. No. TN5-H82F9), at 100 ng/well, can bind to the Human CD40 Ligand, His,Flag Tag, premium grade (Cat. No. CDL-H52Db) with a linear detection range of 20-0.078 μg/mL (QC tested).
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