APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (0.03% Proclin)Nanoflow verified

Flow cytometric analysis of Anti-BCMA CAR (CQ)-293 Stable Cell Line stained with APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-AY2491a) at a 1:50 dilution (2 μL of antibody stock solution was used to stain 1 × 10⁶ cells in a final volume of 100 μL), with an isotype control antibody included as a negative control. APC fluorescence signals was used to evaluate binding activity (QC tested).

Flow cytometric analysis of the non-specific binding of APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-AY2491a) to CD3+ cells in human PBMCs. A total of 5 × 10⁵ human PBMCs were stained with FITC-labeled anti-CD3 antibody alone (left) or co-stained with FITC-labeled anti-CD3 antibody and APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (right) (2 μL of antibody stock solution per 5 × 10⁵ cells in a final staining volume of 100 μL), washed, and analyzed by FACS. FITC and APC fluorescence signals were used to evaluate the non-specific binding of the APC-labeled antibody to human CD3+ cells (QC tested).

After Fc receptor blockade, the whole blood only control (Figure A) and the whole blood spiked with CAR-T cells (Figures B, C, and D) were first stained with anti-CD45 antibody, anti-CD3 antibody, and APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (0.03% Proclin) (Cat. CAH-AY2491a), followed by 7-AAD staining. The data were analyzed using FCS Express 7 software (routinely tested). CAR-T cells were identified as APC-positive events within the live CD45⁺CD3⁺ (or CD45⁺) lymphocyte population. In panels B, C, and D, the proportions of CD45⁺ CAR⁺ cells were approximately 20%, 10%, and 1%, respectively, consistent with the theoretical spike‑in values of 22.16%, 11.38%, and 1.14%.

2-3*10¹⁰ particles/mL of tLNP-hCD8(VHH)-Ab (tLNP Coupling VHH Ab) were stained with APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-AY2491a) using 1:50 dilution, APC signal was used to evaluate the binding activity (Routinely tested).

The binding activity of APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-AY2491a) to different humanized VHHs was evaluated by ELISA. Humanized VHHs were coated at 1 μg/mL. The antibody was serially diluted starting at 125 ng/mL, followed by incubation with an HRP-conjugated goat anti-rabbit IgG Fc-specific secondary antibody at a 1:30,000 dilution. Absorbance was measured at 450 nm (Routinely tested).

The binding activity of APC-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-AY2491a) to different alpaca VHHs was evaluated by ELISA. Alpaca VHHs were coated at 1 μg/mL. The antibody was serially diluted starting at 62.5 ng/mL, followed by incubation with an HRP-conjugated goat anti-rabbit IgG Fc-specific secondary antibody at a 1:30,000 dilution. Absorbance was measured at 450 nm (Routinely tested).