Human IgG

Antibody Internalization Detection Reagent utilizes a VHH single‑domain antibody conjugated to a pH‑sensitive fluorescent dye, specially engineered for acid‑triggered signal amplification. With its smaller molecular weight and low steric hindrance, this reagent specifically binds to the target region of antibodies to form a stable complex, enabling real‑time monitoring of antibody endocytosis in live cells. The pH‑sensitive dye not only significantly enhances the fluorescence signal in acidic endosomal environments but also effectively minimizes background noise, ensuring high sensitivity and accuracy for internalization detection.