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Your Position: > Protein > FcRn (FCGRT & B2M) > FCM-C5284

Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & BLI verified)

  • 동의어
    FcRN,FCGRT & B2M,FCGRT&B2M,B2M,Beta-2 microglobulin,Fcr,Fcgr,fc gamma,fc receptor,Fc gamma receptor,Fc receptor
  • 출처
    Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-C5284), is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1(FCGRT) & Q8SPW0-1(B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are homologus.
    Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
  • 분자 표징
    Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)

    Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284) SDS-PAGE gel

Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ) MALS images

The purity of Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284) is more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on CM5 Chip via anti-His antibody, can bind  Herceptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus Serum Albumin, His,Avitag (Cat. No. HSA-C82E5) on SA Biosensor, can bind Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284) with an affinity constant of 0.684 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 525,000

가격(KRW) : 3,960,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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