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Your Position: > Protein > CD8 alpha > CDA-HA2H4

Alexa Fluor™ 488-Labeled Human CD8 alpha Protein, His TagStar Staining

  • Synonym
    CD8A,CD8,Leu2,MAL,p32
  • Source
    Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (CDA-HA2H4) is produced via conjugation of AF488 to Human CD8 alpha Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD8 alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).
    Predicted N-terminus: Ser 22
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.5 kDa and 15.5 kDa.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Protein Ratio
    The AF488 to protein molar ratio is 0.9-1.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
 CD8 alpha FACS

1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H4) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

 CD8 alpha FACS

1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H4) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

  • Background
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
  • Clinical and Translational Updates

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