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Your Position: > Protein > IL-2 R beta > ILA-C82E3

Biotinylated Cynomolgus IL-2 R beta Protein, His,Avitag™ (MALS verified)

  • Synonym
    IL2RB,RP5-1170K4.6,CD122,P70-75
  • Source
    Biotinylated Cynomolgus IL-2 R beta Protein, His,Avitag(ILA-C82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 (Accession # Q38J85-1).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    IL-2 R beta Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.4 kDa. The protein migrates as 37-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-2 R beta SDS-PAGE

Biotinylated Cynomolgus IL-2 R beta Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-2 R beta MALS images

The purity of Biotinylated Cynomolgus IL-2 R beta Protein, His,Avitag (Cat. No. ILA-C82E3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-2 R beta ELISA

Immobilized Biotinylated Cynomolgus IL-2 R beta Protein, His,Avitag (Cat. No. ILA-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) with a linear range of 0.08-2 ng/mL (QC tested).

  • Background
    Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit.
    CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
  • Clinical and Translational Updates

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