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Your Position: > Protein > Hemagglutinin/HA (Influenza Virus) > HAE-V52H5

Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag

  • Source
    Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (HAE-V52H5) is expressed from human 293 cells (HEK293). It contains AA Asn 18 -Thr 546 (Accession # EPI1843276, GISAID).
    Predicted N-terminus: Asn 18
  • Molecular Characterization
    Hemagglutinin/HA (Influenza Virus) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 59.3 kDa. The protein migrates as 55 kDa and 85-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Hemagglutinin/HA (Influenza Virus) SDS-PAGE

Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Hemagglutinin/HA (Influenza Virus) ELISA

Immobilized Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H5) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Influenza B Hemagglutinin / HA Antibody, Rabbit Mab (QC tested).

 Hemagglutinin/HA (Influenza Virus) ELISA

Immobilized Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HA (B/Phuket/3073/2013) Monoclonal Antibodywith a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
  • Clinical and Translational Updates

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