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Your Position: 홈 > Antibody > TNF-alpha > TNA-BLM494

Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Source
    Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with TNF-alpha.
  • Isotype
    Human IgG1 | Human Kappa
  • Specificity
    This product is a specific antibody specifically reacts with TNF-alpha.
  • Application
    ELISA,WB
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Endotoxin
    Less than 1.0 EU per mg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TNF-alpha SDS-PAGE

Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
TNF-alpha MALS images

The purity of Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) is more than 90% and the molecular weight of this protein is around 160-185 kDa verified by SEC-MALS.

Report
Bioactivity-Elisa
TNF-alpha ELISA

Immobilized Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) at 2 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) with a linear range of 0.20-3.13 ng/mL (QC tested).

Protocol
TNF-alpha ELISA

Immobilized Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (13B8) (Cat. No. TNA-AM493) at 2 μg/mL, add increasing concentrations of Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228), and then add Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) at 0.25 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.02 ng/mL (Routinely tested).

Protocol
Bioactivity-SPR
TNF-alpha SPR

Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 75.9 pM as determined in a SPR assay (Biacore 8K) (Routinely  tested).

Protocol
Bioactivity-BLI
TNF-alpha BLI

Loaded Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 2.13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
TNF-alpha BLI

Loaded Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) on Protein A Biosensor, can bind Human TNF-alpha, premium grade (Cat. No. TNA-H4211) with an affinity constant of 3.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Background
    Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
  • Clinical and Translational Updates

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