로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
Promotion
Recombinant Proteins ClearClear history
Recombinant Proteins CloseClose
Browse Products By Molecule Name a-zA-Z
TL1A Hot |GMP IL-2 Hot |Claudin 18.2 Hot |FMC 63 Ab  |FcRn
0
장바구니가 비어 있습니다
내 장바구니
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: 홈 > Kits > BCMA > EP-118

BCMA [Biotinylated] : BAFF Inhibitor Screening ELISA Kit

For research use only.
IDComponentsSize
EP118-C01High-bind Plate1 plate
EP118-C02Human BAFF10 μg
EP118-C03Biotinylated Human BCMA10 μg
EP118-C04Anti-BCMA Neutralizing Antibody20 μg
EP118-C05Streptavidin-HRP5 μg
EP118-C06Coating Buffer12 mL
EP118-C0710xWashing Buffer 50 mL
EP118-C08Blocking Buffer50 mL
EP118-C09Substrate Solution12 mL
EP118-C10Stop Solution7 mL
  • Background
    TNFRSF17 is a member of the TNF-receptor family, known as tumor necrosis factor receptor superfamily member 17, also known as B cell maturation antigen (BCMA or BCM) or CD269. It is a protein encoded by TNFRSF17 gene.  The receptor is expressed on the surface of B lymphocytes and plasma cells and is a marker protein of B lymphocyte maturation.The main ligands of BCMA are BAFF and APRIL. BCMA combined with BAFF could activate the classical and non-classical NF-κB and JNK signaling pathways, up-regulate the expression of anti-apoptotic protein and down-regulate the expression of pro-apoptotic protein. When BCMA binds to APRIL, the expression level of immune checkpoint is up-regulated, creating an immunosuppressed bone marrow microenvironment.
  • Application

    This kit is developed for screening for inhibitors of human BCMA binding to human BAFF.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening Assay kit is designed to facilitate the identification and characterization of new BCMA pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human BCMA to immobilized human BAFF in a functional chemiluminescence assay. Briefly, we provide you with a human Biotinylated BCMA protein, a human BAFF protein, an anti-BCMA neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human BAFF.

    b) Add your molecule of interest to the tests.

    c) Add human BCMA-Biotin to bind the coated human BAFF.

    d) Add Streptavidin-HRP followed by luminol .

    Finally, the half maximal inhibitory concentration (IC50) of your compound to BCMA: BAFF binding will be determined by comparing RLU readings among different experimental groups.

Typical Data Please refer to Ds document for the assay protocol.
BCMA TYPICAL DATA

INHIBITION OF BCMA [BIOTINYLATED]: BAFF BINDING BY ANTI-BCMA NEUTRALIZING ANTIBODY
Serial dilutions of Anti-BCMA Neutralizing antibody (Catalog # EP118-C04) (1:1 serial dilution, from 10 μg/mL to 0.0048828125μg/mL) was added into BAFF: Biotinylated BCMA binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 975,000

장바구니에 담기 대량구매 맞춤형 제품

Antibody Licensing

more

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs：7 Details
  • Number of Drugs in Clinical Trials：101 Details
  • Latest Research Phase：Approved
다른 추천 제품

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)
Show All Distributors

관련 제품

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop
Call us
우리에게 전화하세요
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Email
이메일
order.kr@acrobiosystems.com
Fax
팩스
+1 888-377-6111
Address
주소
서울 성동구 왕십리로10길 6 서울숲비즈포레 703
회사명
아크로바이오 주식회사
사업자 등록번호
186-88-02858
대표자
CHEN YIDING
사업장주소
서울특별시 송파구 중대로12길 10-20, 1층 셀렉티드송파(가락동)
linkedin twitter facebook youtube

문의

Copyright © 2017 ACROBiosystems. All Rights Reserved
Related Links|Privacy Policy|Terms and Conditions|Legal|Sitemap