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Your Position: > CKP > Streptavidin > STN-NA114

Streptavidin-Acridine ester (MALS verified)

  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin-Acridine ester (STN-NA114) is Acridine ester chemically conjugated Streptavidin expressed from E.coli cells.
  • Molecular Characterization

    Streptavidin carries no "tag". The protein has a calculated MW of 13.8 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Streptavidin SDS-PAGE

Streptavidin-Acridine ester on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Streptavidin MALS images

The purity of Streptavidin-Acridine ester (Cat. No. STN-NA114) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Protein G Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).

 Streptavidin MPCLIA

Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Anti-Human IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

가격(KRW) : 36,000

가격(KRW) : 270,000

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