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Your Position: > Protein > Axl > AXL-H82F9

Biotinylated Human AXL Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    AXL,UFO
  • Source
    Biotinylated Human AXL, Fc,Avitag(AXL-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Trp 451 (Accession # P30530-1).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    Axl Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 74.3 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Axl SDS-PAGE

Biotinylated Human AXL, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Axl MALS images

The purity of Biotinylated Human AXL, Fc,Avitag (Cat. No. AXL-H82F9) is more than 90% and the molecular weight of this protein is around 155-185 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Axl ELISA

Immobilized Human GAS6, His Tag (Cat. No. GA6-H5249) at 5 μg/mL (100 μL/well) can bind Biotinylated Human AXL, Fc,Avitag (Cat. No. AXL-H82F9) with a linear range of 1-31 ng/mL (QC tested).

  • Background
    AXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.
  • Clinical and Translational Updates

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