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Your Position: > Viral Proteins > Spike RBD > SPD-C82Ep

Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free) (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • 출처
    Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free) (SPD-C82Ep) is expressed from human 293 cells (HEK293).
  • 분자 표징

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.3 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

  • 레시피

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 .

    Contact us for customized product form or formulation.

  • 보관

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
    2. For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
  • 배송
    This product is supplied and shipped as sterile liquid solution with blue ice, please inquire the shipping cost.
SDS-PAGE
Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free)  (Cat. No. SPD-C82Ep) SDS-PAGE gel

Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free)  (Cat. No. ) MALS images

The purity of Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag™ (Trehalose free) (Cat. No. SPD-C82Ep) is more than 85% and the molecular weight of this protein is around 30-44 kDa verified by SEC-MALS.

  • 백그라운드
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

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