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Your Position: > Agonist > CD40 Ligand > CDL-M82H5

Biotinylated Mouse CD40 Ligand / TNFSF5 Protein, His,Avitag™ (active trimer) (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
  • 출처
    Biotinylated Mouse CD40 Ligand, His,Avitag (CDL-M82H5) is expressed from human 293 cells (HEK293). It contains AA Gly 115 - Leu 260 (Accession # P27548-1).
    Predicted N-terminus: His
  • 분자 표징
    Online(Gly 115 - Leu 260) P27548-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 19.6 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) SDS-PAGE gel

Biotinylated Mouse CD40 Ligand, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) is more than 90% and the molecular weight of this protein is around 56-68 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse CD40 Ligand, His,AvitagBiotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) ELISA bioactivity

Immobilized Mouse CD40, His Tag (Cat. No. TN5-M52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) with a linear range of 0.5-16 ng/mL (QC tested).

  • 백그라운드
    CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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