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Your Position: > Antibody > Adalimumab > ADB-Y23b

Anti-Adalimumab Antibody (AY23b) (recommended for PK/PD)

  • Source
    Anti-Adalimumab Antibody (AY23b) (ADB-Y23b) is expressed from human 293 cells.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Adalimumab SDS-PAGE

Anti-Adalimumab Antibody (AY23b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Adalimumab MALS images

The purity of Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity-Elisa
 Adalimumab ELISA

Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) with a linear range of 0.2-3 ng/mL (QC tested).

 Adalimumab ELISA

Immobilized Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) at 2 μg/mL, add increasing concentrations of Adaliumumab and then add Biotinylated Biotinylated Anti-Adalimumab Antibody (AY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (QC tested).

  • Background
    Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 450,000

가격(KRW) : 3,270,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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