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HEK293/Human ACE2 Stable Cell Line

  • Description
    This HEK293 cell line is engineered to stably overexpress human angiotensin I converting enzyme 2 (ACE2) and is highly permissive to infection by lentiviral particles pseudotyped with SARS-COV-2 spike protein. The HEK293/Human ACE2 Overexpression Stable Cell Line is a powerful tool for in vitro screening and characterization of potential drugs against SARS-COV-2.
  • Application

    Screening of small molecule inhibitors and/or neutralizing antibodies of the ACE2‑Spike interaction

  • Growth Properties
    Adherent
  • Selection Marker
    NA
  • Culture Medium
    90% DMEM high glucose + 10% FBS
  • Freeze Medium
    10% FBS+10% DMSO+80% DMEM high glucose
  • Quantity
    1 vial contains ~ 1x10^6 cells in 1 ml of 10% DMSO in (DMEM+10%FBS).
  • Storage
    Frozen in liquid nitrogen.
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Bioactivity
 ACE2 FACS

Flow Cytometry assay shows that SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) can bind to HEK293/Human ACE2 Overexpression Stable Cell Line (Cat. No. CHEK-ATP042). The concentration of S1 used is 20 μg/mL (QC tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 3,570,000

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