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Your Position: > Agonist > CD40 Ligand > CDL-H82Db

Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,His,Flag Tag (active trimer) (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • Synonym
    CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
  • Source
    Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,His,Flag Tag (CDL-H82Db) is expressed from human 293 cells (HEK293). It contains AA Gly 116 - Leu 261 (Accession # P29965-1 ).
    Predicted N-terminus: Gly
  • Molecular Characterization

    This protein carries a Avi tag (Avitag™) at the N-terminus, followed by flag tag. The polyhistidine tag is inserted in-between the Avi tag and Flag tag

    The protein has a calculated MW of 51.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD40 Ligand SDS-PAGE

Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD40 Ligand MALS images

The purity of Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,His,Flag Tag (Cat. No. CDL-H82Db) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD40 Ligand ELISA

Immobilized Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. CD0-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (Cat. No. CDL-H82Db) with a linear range of 0.1-2 ng/mL (QC tested).

 CD40 Ligand ELISA

Immobilized Biotinylated Human CD40 Ligand Protein, Avitag,His,Flag Tag (Cat. No. CDL-H82Db) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD40 Protein, Fc Tag (MALS verified) (Cat. No. CD0-H5253) with a linear range of 0.5-8 ng/mL (Routinely tested).

  • Background
    CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.
    CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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