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Your Position: > Antibody > FMC63 > FM3-FY45

FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45)DMF

  • Source
    FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with FMC63.
    We carry another premium grade FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-FY45G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1-2.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
    *The Isotype control (Cat. No. DNP-FM1A1) is sold separatelyand you can follow this link for product information.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Bioactivity-FACS
 FMC63 FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-FY45) and isotype control respectively. FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 430,500

가격(KRW) : 2,673,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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