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Your Position: > Protein > OX40 > OX0-M82E5

Biotinylated Mouse OX40 / TNFRSF4 / CD134 Protein, His,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • 동의어
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • 출처
    Biotinylated Mouse OX40, His,Avitag (OX0-M82E5) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 211 (Accession # P47741-1).
    Predicted N-terminus: Val 20
  • 분자 표징
    Online(Val 20 - Pro 211) P47741-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 25.0 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse OX40, His,Avitag (Cat. No. OX0-M82E5) SDS-PAGE gel

Biotinylated Mouse OX40, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Mouse OX40, His,AvitagBiotinylated Mouse OX40, His,Avitag (Cat. No. OX0-M82E5) ELISA bioactivity

Immobilized Mouse OX40 Ligand, His Tag (Cat. No. TN4-M5241) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse OX40, His,Avitag (Cat. No. OX0-M82E5) with a linear range of 0.6-10 ng/mL (QC tested).

  • 백그라운드
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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