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ELISA Assay Kit for Anti-CTLA-4 h-mAb in Mouse Serum

For research use only.
  • Background
    How a therapeutic antibody is metabolized in the body is pertinently relevant to its efficacy. Therefore, pharmacokinetics study is an important part of the drug development. CTLA-4 is probably the most targeted molecule in today’s pharmaceutical industry, thanking to the clinical success of Yervoy®. Many investigational anti-CTLA-4 mAbs are being developed. There is a growing need for a standard assay that can be used to facilitate the study of their pharmacokinetics.
  • Application
    It is for research use only.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store all items at -20°C. After reconstitution, the stock solution should be kept at -80°C.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20°C for 1 year in lyophilized state; -80°C for 4 months under sterile conditions after reconstitution.
  • Assay Principles
    The enclosed ELISA assay kit for anti-CTLA-4 h-mAb in mouse serum is based on an enzyme immunoassay (ELISA) between recombinant CTLA-4 protein (ECD) and a biotinylated anti-CTLA-4 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-CTLA-4 antibodies in mouse serum.

    The assay only involves the following steps:

    1. Coat the plate with human CTLA-4;

    2. Add the mixture of your sample and biotinylated anti-CTLA-4 antibody;

    3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

    4. Record the OD readings and analyze the serum concentration.

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ETD of in-stock products: 4 business days

가격(KRW) : 474,000

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