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Your Position: > Protein > CD27 Ligand > CDL-M5251

Mouse CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag(CDL-M5251) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
    Predicted N-terminus: Glu
  • Molecular Characterization
    CD27 Ligand Structure

    This protein carries a mouse IgG2a Fc tag at the N-terminus

    The protein has a calculated MW of 43.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD27 Ligand SDS-PAGE

Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 CD27 Ligand ELISA

Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 1 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-M5251) with a linear range of 0.3-2 ng/mL (QC tested).

  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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