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Your Position: > Protein > FcRn (FCGRT & B2M) > FCM-M82W6

Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Twin-Strep Tag (MALS & BLI-verified)

  • 동의어
    FcRn,FCGRT & B2M
  • 출처
    MABSol® Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1(FCGRT) & P01887-1(B2M)).
    Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
  • 분자 표징
    Online(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559-1 (FCGRT) & P01887-1 (B2M)

    Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) SDS-PAGE gel

Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. ) MALS images

The purity of Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) on SA Biosensor, can bind Herceptin with an affinity constant of 3.38 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • 백그라운드
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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약물 개발 현황

  • Number of Launched Drugs:3 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

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DMF 신청
DMF (Drug Master File)

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