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Your Position: > Signal Transduction > EGF R > EGR-C52H1

Rhesus macaque EGF R Protein, His Tag

  • 동의어
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • 출처
    Rhesus macaque EGF R, His Tag (EGR-C52H1) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P55245).
    Predicted N-terminus: Leu 25
  • 분자 표징
    Online(Leu 25 - Ser 645) P55245

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rhesus macaque EGF R, His Tag (Cat. No. EGR-C52H1) SDS-PAGE gel

Rhesus macaque EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Rhesus macaque EGF R, His TagRhesus macaque EGF R, His Tag (Cat. No. EGR-C52H1) ELISA bioactivity

Immobilized Rhesus macaque EGF R, His Tag (Cat. No. EGR-C52H1) at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.2-3 ng/mL (QC tested).

  • 백그라운드
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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