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Your Position: > Protein > B7-H5 > B75-H52H0

Human B7-H5 / Gi24 / VISTA Protein, His Tag (MALS verified)

  • 동의어
    B7-H5,SISP1,Gi24,VISTA
  • 출처
    Human B7-H5, His Tag (B75-H52H0) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568).
    Predicted N-terminus: Phe 33
  • 분자 표징
    Online(Phe 33 - Ala 194) AAH20568

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.0 kDa. The protein migrates as 33-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H5, His Tag (Cat. No. B75-H52H0) SDS-PAGE gel

Human B7-H5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human B7-H5, His Tag (Cat. No. ) MALS images

The purity of Human B7-H5, His Tag (Cat. No. B75-H52H0) is more than 95% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human VSIG3, Fc Tag (Cat. No. VS3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human B7-H5, His Tag (Cat. No. B75-H52H0) with an affinity constant of 53.4 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human B7-H5, His Tag (Cat. No. B75-H52H0) on HIS1K Biosensor, can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 23 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human VSIG3, Fc Tag (Cat. No. VS3-H5258) on AHC Biosensor, can bind Human B7-H5, His Tag (Cat. No. B75-H52H0) with an affinity constant of 16 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • 백그라운드
    Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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재고상품 출발날짜: 영업일 기준 4일

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  • Number of Drugs in Clinical Trials:6 Details
  • Latest Research Phase:Phase 2 Clinical

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